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By Robert K. Ockner (auth.), Jan F. C. Glatz, Ger J. Van Der Vusse (eds.)

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26. synthetic oligonucleotide probe. Neurosci Lett 58: 101-105, 1985 Uhl GR, Zingg HH, Habener JF: Vasopressin mRNA in situ hybridization: localization and regulation studied with oligonucleotide cDNA probes in normal and Brattleboro rat hypothalamus. Proc Natl Acad Sci USA 82: 5555-5559, 1985 Priestley JV, Hynes MA, Han VKM, Rethelyi M, Perl ER, Lund PK: In situ hybridization using 32p labelled oligodeoxyribonucleotides for the cellular localisation of mRNA in neuronal and endocrine tissue. An analysis of procedural variables.

N° 1188), Universite de Paris V, 45 rue des Saints-Peres, F 75270 Paris Cedex 06, France Key words: amino acid sequence, bovine, brain, binding Summary A fatty acid-binding protein (FABP) from the cytosol of bovine brain was purified by Sephadex G-75 filtration and electrofocusing. 7 kDa. To ascertain that the purified protein was a FABP, it was submitted to fatty acid-binding tests. Oleic and palmitic acids bound to brain FABP but this was not the case for palmitoyl CoA. 1 for palmitic acid. The complete amino acid sequence of the brain F ABP was determined and a microheterogeneity was observed.

18. 19. 20. AV, Brecher P: Properties and differential regulation of two fatty acid-binding proteins in the rat kidney. J Bioi Chern 263: 15762-15768,1988 Schoentgen F, Pignede G, Bonanno LM, Jolles P: Fattyacid-binding protein from bovine brain. Amino acid sequence and some properties. Eur J Biochem 185: 35-40, 1989 Boersma WJA, Claassen E, Deen C, Gerritse K, Haaijman JJ, Zegers ND: Antibodies to short synthetic peptides for specific recognition of partly denatured protein. Anal Chim Acta 213: 187-197, 1988 Alexander HD, Johnson DA, Rosen J, Jerabek L, Green N, Weissman IL, Lerner RA: Mimicking the alloantigenicity of proteins with chemically synthesized peptides differing in single amino acids.

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